Title: Assessment of the possibility of seed propagation of microbubbles and plants in vitro in field conditions.

Financing institution: National Science Center

Implementation period: 2010-2012

Main performer: mgr. Eng. Agnieszka Barnyk

Contractors: dr that. Sławomir Wróbel – project manager

prof. dr hab. M. Kostiw – contractor

Implementing unit: Department of Seed Science and Potato Protection IHAR-PIB in Bonin

Project value: PLN 250,000

Purpose of research:

Field tests carried out on two potato varieties with differentiated virus resistance were aimed at:

  • Comparison of PVY, PVM and PLRV infestation of daughter tubers grown from in vitro plants, microbubbles, mini tubers and traditional seed potatoes.
  • Assessment of the impact of three in vitro planting dates and microbubbles in field conditions on the level of infection of daughter tubers with the above viruses.
  • Comparison of in vitro production of plants, microbubbles, minibubes and traditional seed potatoes.

The main purpose of laboratory tests, in addition to the annual assessment of infestation of collected tuber samples from field experiments in the mesh sample test DAS-ELISA, was to determine the susceptibility of plants grown from various forms of seed materials (in vitro plants, microbubbles, mini tubers, traditional seed potatoes) to PVY, PVM and PLRV infection under artificial inoculation with aphids Myzus persicae and Aphis nasturtii.

Results achieved:

  • Survival in field conditions of plants grown from microbubbles and in vitro plants was highly dependent on the prevailing weather conditions. Low temperatures, strong winds and drought had adverse effects.
  • In vitro propagation of microbubble and plant in vitro varieties of relatively resistant to viruses can be very profitable.
  • Subsequent planting (end of June) and the use of an additional cover in the form of agrotextile in the first period of plant growth allows microbubble and plant in vitro cultivation of varieties with low resistance to viruses.
  • It is difficult to unequivocally explain the increased susceptibility of microbubbles and plants in vitro to viral infections in the field.
  • It seems that the reason for the higher paralysis is not their delicacy, but another, not fully understood cause, therefore this issue requires further research.