Research projects financed from the Program of the Ministry of Agriculture and Rural Development – Biological Progress in Plant Production:
Title: Development of sensitive methods for detection of major potato viruses
Financing institution: Ministry of Agriculture and Rural Development, PBwPR program
Implementation period: 2014-2020
Project manager: dr Krzysztof Treder
Implementing unit: Department of Seed Science and Potato Protection IHAR-PIB in Bonin
Project value: PLN 2,106,000.00.
Purpose of research:
The aim of the project is: (I) development and optimization of new methods of detection of viruses directly in tubers and sprouts, (II) assessment of the impact of potato varieties resistance on detection efficiency of tubers (III) assessment of germ suitability for virus detection, (IV) adaptation and optimization molecular methods for detecting viruses in plants in vitro, and (V) development of rapid molecular tests suitable for detecting viruses in field conditions.
Results achieved:
Microspheres with good magnetic and dispersion properties were produced.
A magnetic variant of the ELISA test was developed.
Sensitive primers for detecting PLRV by RT-PCR have been developed.
Sensitive primers for PVY detection by isothermal nucleic acid amplification – RT-LAMP have been developed.
Publications:
Przewodowska A., Zacharzewska B., Chołuj J., Treder K. 2015. A one-step, real-time reverse transcription loop-mediated isothermal amplification assay to detect Potato virus Y. American Journal of Potato Research. Doi: 10.1007 / s12230-015-9430-3.
Zacharzewska B., Przewodowska A., Treder K. 2014. The adaptation of silica capture RT-PCR for the detection of Potato virus Y. American Journal of Potato Research, 91: 525-531.
Zacharzewska B., Treder K. 2014. ELISA and its modifications. Potato Polish, 24 (1).